CELL CULTURE

L-929 Conditioned Medium

PEI Transfection

OTHERS

Isolation of Peripheral Blood Leukocytes by Ficoll Gradient Centrifugation

BrdU Preparation and Injection

Immunprecipitation with Dynabeads

GENOTYPING

Foxd1GCE

Spry1 LacZ

Foxd1GC

Frizzled4

ATG7-flox

ATG5-flox

Mouse Tail Tips-Preparation

CD11b-DTR

EVI-flox

IL-2 Control

Col4a3 KO (Alport)

IL-10 GFP

BAT-gal

Tie2 GFP

iDTR

Csf1R-iCre

MyD88-flox

CTGF-flox

Dicer-flox

TdTomato

dsRed

DTA

General Cre

IMMUNOSTAINING

X-Gal Beta-Galactosidase Staining

General Immunofluorescence Staining

Sirius Red Staining For Collagen

WESTERN BLOT

Ponceau S

SDS-PAGE Running Buffer

TBS:TBS-T

MOLECULAR BIOLOGY

Neutral Southern with Alkaline Transfer

DNA Extraction from Palmiter's Lab

Phenol Chloroform Extraction

Screening bacterial colonies Protocol

Southern Blot

Preparation of DNA for Electroporation of ES cells

Alkaline Lysis

Real Time PCR Machine and Software

LAB STOCKS

FACS solutions

PFA

Sodium Orthovanadate (Tyrosine Phosphatase Inhibitor)

50x TAE

TBS:TBS-T

TE Buffer

10x TBE

DNA loading buffers

PBS

PLP fixative