CELL CULTURE

L-929 Conditioned Medium

PEI Transfection

GENOTYPING

BAT-gal

IL-10 GFP

Dicer-flox

Foxd1GC

CD11b-DTR

Csf1R-iCre

dsRed

DTA

EVI-flox

CTGF-flox

IL-2 Control

iDTR

Spry1 LacZ

Mouse Tail Tips-Preparation

Tie2 GFP

ATG7-flox

Col4a3 KO (Alport)

TdTomato

Frizzled4

Foxd1GCE

MyD88-flox

General Cre

ATG5-flox

IMMUNOSTAINING

General Immunofluorescence Staining

X-Gal Beta-Galactosidase Staining

Sirius Red Staining For Collagen

LAB STOCKS

DNA loading buffers

50x TAE

FACS solutions

PFA

PLP fixative

TBS:TBS-T

10x TBE

TE Buffer

Sodium Orthovanadate (Tyrosine Phosphatase Inhibitor)

PBS

MOLECULAR BIOLOGY

Alkaline Lysis

Phenol Chloroform Extraction

Real Time PCR Machine and Software

Southern Blot

Screening bacterial colonies Protocol

DNA Extraction from Palmiter's Lab

Neutral Southern with Alkaline Transfer

Preparation of DNA for Electroporation of ES cells

OTHERS

Immunprecipitation with Dynabeads

BrdU Preparation and Injection

Isolation of Peripheral Blood Leukocytes by Ficoll Gradient Centrifugation

WESTERN BLOT

SDS-PAGE Running Buffer

Ponceau S

TBS:TBS-T