ATG7 Mice Genotyping

Forward: 5'-TGG CTG CTA CTT CTG CAA TGA TGT-3'
Reverse: 5'-CAG GAC AGA GAC CAT CAG CTC CAC-3'

 

Genomic DNA                    1 μL

2x PCR Mix                         10 μL

Forward                              1 μL

Reverse                                1 μL

Water                                   7 μL

 

Total                                      20 μL

 

 

94°C                          5 minutes

30 cycles:

94°C                          20 seconds

68°C                          1 minute 30 seconds

 

72°C                          7 minutes

4°C