BAT-gal Mice Genotyping

 

Genomic DNA                                1 μL

2X Master Mix                               10 μL

oligo #198 (BAT-gal F)              1 μL

oligo #199 (BAT-gal R)              1 μL

oligo # 112 (IL2-F)                      1 μL

oligo #113 (IL2-R)                      1 μL

Water                                               5 μL

 

Total                                                  20 μL

 

 

94°C                          3 minutes

35 cycles:

94°C                          30 seconds

59°C                          60 seconds

72°C                          60 seconds

 

72°C                          5 minutes

4°C                             ∞

 

Transgene: ~200 bp

IL-2 control: ~324 bp