CD11b-DTR Mice Genotyping 

 

Reaction Mixture

 

Genomic DNA                    1 μL

2X PCR Mix                         10 μL

oligo #160                          1 μL

oligo #161                          1 μL

Water                                   7 μL

 

Total                                      20 μL

 

 

PCR Mixture

 

94°C                          5 min

35 cycles:

94°C                          30 seconds

51°C                          30 seconds

72°C                          75 seconds

 

72°C                          5 min

4°C                            

 

Positive band: ~1000 bp