Csf1R-iCre Mice Genotyping

 

Genomic DNA                    1 μL

2X Master Mix                   10 μL

oligo #285                          1 μL 

oligo #286                          1 μL

Water                                   7 μL

 

Total                                      20 μL

 

94°C                          3 minutes

 

35 cycles:

94°C                          45 seconds

60°C                          45 seconds

72°C                          45 seconds

 

72°C                          5 minutes

4°C                            

 

Positive band: ~392 bp