DTA (Gt(ROSA)26Sortm1Sor) Genotyping

 

Genomic DNA                                1 μL

2X Master Mix                               10 μL

Oligo #459                                      1 μL

Oligo #460                                      1 μL

Oligo #461                                      1 μL

Water                                               6 μL

 

Total                                                  20 μL

 

 

94íC                          1 minute 30 seconds

35 cycles

94íC                          30 seconds

62íC                          1 minute

72íC                          1 minute

 

72íC                          2 minutes

4íC                            

 

 

Wild type band: ~550 bp

Mutant band: 300 bp

Heterozygote: 300 bp and ~550 bp