Dicer Mice Genotyping

 

Genomic DNA                    1 μL

2X Master Mix                   10 μL

Oligo #563                          1 μL

Oligo #564                          1 μL

Water                                   7 μL

 

Total                                      20 μL

 

 

94°C                          3 minutes

35 cycles:

94°C                          30 seconds

55°C                          30 seconds

72°C                          30 seconds

 

72°C                          5 minutes

4°C                            

 

 

Mutant band:  ~420 bp

Wild type band: ~351 bp

Heterozygote: ~351 bp and ~420 bp