Foxd1-GFP-Cre (Foxd1-GC) Mice Genotyping

 

Genomic DNA                    1 μL

2X Master Mix                   10 μL

Oligo #861                          1 μL

Oligo #862                          1 μL

Oligo #863                          1 μL

Water                                   6 μL

 

Total                                      20 μL



94C                          3 minutes

35 cycles:

94C                          30 seconds

59C                          30 seconds

72C                          30 seconds

 

72C                          2 minutes

4C                            

 

 

Foxd1-GC band: ~450 bp

Wild type band: ~250 bp