Foxd1GCE Mice Genotyping

 

Genomic DNA                    1 μL

2X PCR Mix                         10 μL

Oligo #430                          1 μL

Oligo #431                          1 μL

Water                                   7 μL

 

Total                                      20 μL

 

 

94C                          5 minutes

38 cycles:               

94C                          45 seconds

65C                          45 seconds

72C                          1 minute

 

72C                          10 minutes

4C                            

 

 

Positive band: 445 bp