Frizzled4 Mice Genotyping

 

 

Wild Type Allele

 

Genomic DNA                    1 μL

2X Master Mix                   10 μL

oligo #296                          1 μL

oligo #297                          1 μL

Water                                   7 μL  

 

Total                                      20 μL

 

 

Knock-Out Allele

 

Genomic DNA                    1 μL

2X Master Mix                   10 μL

oligo #303                          1 μL

oligo #304                          1 μL

Water                                   7 μL              

 

Total                                      20 μL

 

 

94                         3 minutes

40 cycles:

94                         30 seconds

49                         30 seconds

72                         60 seconds

 

72                         5 minutes

4íC                            

 

Wild type allele:   434 bp

KO allele: ~400 bp