Cre Mice Genotyping

 

Genomic DNA                    1 μL

2X  PCR Mix                        10 μL

oligo # 129                         1 μL

oligo #130                          1 μL

Water                                   7 μL

 

Total                                      20 μL

 

 

94°C                          3 minutes

35 cycles:

94°C                          45 seconds

63°C                          45 seconds

72°C                          60 seconds

 

72°C                          5 minutes

4°C                              ∞

 

 

Positive band: ~420bp