IL-2 Control Genotyping 

 

Genomic DNA                                 1 μL

2X Master Mix                                10 μL

oligo #112 (IL2-F)                        1 μL

oligo #113 (IL2-R)                       1 μL

Water                                                7 μL

 

Total                                                   20 μL 

 

 

94C                     3 minutes 

35 cycles:

94C                     30 seconds

59C                     60 seconds

72C                     60 seconds 

 

72C                     5 minutes

4C                         

 

IL-2 band: 324 bp