MyD88tm1Defr Mice Genotyping

Genomic DNA                                1 μL

2X PCR Mix                                     10 μL

Oligo #569                                      1 μL

Oligo #570                                      1 μL

Water                                               7 μL

 

Total                                                  20 μL

 

 

94C                          3 minutes

35 cycles:

94C                          30 seconds

60C                          1 minutes

72C                          1 minutes

 

72C                          2 minutes

4C                            

 

Mutant band: 353 bp

Wild type band: 266 bp

Heterozygote: 266 bp and 353 bp