Spry1 Lac Z Mice Genotyping

 

Primer Sequences

 Oligo #786 -- Spry 1lacZ for WT: 5' - CAC TAC TTC AAG AAA AGC AAG GTG ACT C-3'
 Oligo #787 -- Spry 1lacZ for mutant: 5' - GCG ACT TCC AGT TCA ACA TC - 3'
 Oligo #788 -- Spry1 lacZ Rev Common: 5' - GGA GAC CAT ACA ACT CTG ACT ACA GTA CTT TCC- 3'

 

Reaction Mixture

 Genomic DNA       1 μL
 2X Master Mix      12.5 μL
 Oligo #788             1 μL

 Oligo #786             0.5 μL

 Oligo #787             0.5 μL

 Water                      9.5 μL

 

 Total                       25 μL

 

 

PCR Program

 Annealing temp: 54°C

 Mutant lacZ band: 520 bp

 Wild type band: 250 bp