CAG-Td Tomato (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) Mice Genotyping

 

Genomic DNA                    2 μL

2X Master Mix                   10 μL

oligo #700                          1 μL

oligo #701                          1 μL

oligo #702                          1 μL

oligo #703                          1 μL

Water                                   4 μL

 

Total                                      20 μL

 

 

94oC               3 minutes

35 cycles:

94oC               20 seconds

61oC               30 seconds

72oC               30 seconds

 

72oC               2 minutes

4íC                

 

Mutant band: 196 bp

Wild type band: 297 bp