Tie2 GFP mice Genotyping

 

Genomic DNA                                            1 μL

2X Master Mix                                           10 μL

oligo #248 (OIMR1263)                        1 μL

oligo #249 (OIMR1264)                        1 μL

oligo #112 (IL2-F)                                   1 μL

oligo #113 (IL2-R)                                  1 μL

Water                                                           5 μL

 

Total                                                              20 μL

 

 

94íC                          1 minute 30 secs

35 cycles:

94íC                          30 seconds

55íC                          1 minute

72íC                          1 minute

 

72íC                          2 minutes

4íC                            

 

Transgene band: 567 bp

IL2 band: 324 bp