Floxed dsRed Mice Genotyping

 

Genomic DNA                    1 μL

2X Master Mix                   10 μL

oligo #171                          1 μL

oligo #172                          1 μL

Water                                   7 μL  

 

Total                                      20 μL

 

94°C                          3 minutes

35 cycles:

94°C                          45 seconds

57°C                          60 seconds

72°C                          60 seconds

 

72°C                          5 minutes

 

Positive band: ~500bp