iDTR (Gt(ROSA)26Sortm1(HBEGF)Awai) Mice Genotyping

 

Genomic DNA                                1 μL

2X Master Mix                               10 μL

Oligo #462                                      1 μL

Oligo #463                                      1 μL

Oligo #464                                      1 μL

Water                                               6 μL

 

Total                                                  20 μL

 

 

94íC                          3 minutes

35 cycles

94íC                          30 seconds

61íC                          1 minute

72íC                          1 minute

 

72íC                          2 minutes

4íC                            

 

 

Wild type band: 603 bp

Mutant band: 242 bp

Heterozygote: 242 bp and 603 bp