BrdU Preparation and Injection

 

BrdU is stored as a powder in the small -20C freezer.

 

It is best to use BrdU when freshly prepared.

 

BrdU should be administered IP 2 hours before harvest, at dose of 50ug/g.

 

Prepare BrdU in 0.9% NaCl at 5mg/mL. Keep warm (~37-50C) and stir until dissolved. Filter. Keep on ice and protect from light.

 

Inject 10uL per gram IP.

 

Example:

 

For weight of 20g, you need 1mg of BrdU. From 5mg/mL stock, inject 200uL.