BrdU Preparation and Injection


BrdU is stored as a powder in the small -20C freezer.


It is best to use BrdU when freshly prepared.


BrdU should be administered IP 2 hours before harvest, at dose of 50ug/g.


Prepare BrdU in 0.9% NaCl at 5mg/mL. Keep warm (~37-50C) and stir until dissolved. Filter. Keep on ice and protect from light.


Inject 10uL per gram IP.




For weight of 20g, you need 1mg of BrdU. From 5mg/mL stock, inject 200uL.