BrdU Preparation and Injection
BrdU is stored as a powder in the small -20C freezer.
It is best to use BrdU when freshly prepared.
BrdU should be administered IP 2 hours before harvest, at dose of 50ug/g.
Prepare BrdU in 0.9% NaCl at 5mg/mL. Keep warm (~37-50C) and stir until dissolved. Filter. Keep on ice and protect from light.
Inject 10uL per gram IP.
For weight of 20g, you need 1mg of BrdU. From 5mg/mL stock, inject 200uL.