SDS-PAGE Running Buffer

(0.025 M Tris, 0.192 M Glycine, 0.1% SDS, pH 8.3)


Add components one at a time to 3.5 L ddH2O:

30.28 g Tris Base (121.1 g/mol)

57.7 g Glycine (75.07 g/mol)

4 g Sodium Dodecyl Sulfate (Measure in chemical hood -- SDS is an irritant)


Adjust pH to 8.3. Bring volume to 4 L with ddH2O.



Store at room temperature for up to one month.